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u6 promoter expresses sgrna  (Addgene inc)


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    Addgene inc u6 promoter expresses sgrna
    U6 Promoter Expresses Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u6+promoter+expresses+sgrna/pm41887225-555-59-58?v=Addgene+inc
    Average 95 stars, based on 103 article reviews
    u6 promoter expresses sgrna - by Bioz Stars, 2026-07
    95/100 stars

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    AcrIIA11 inhibits SaCas9 in vitro and in human HEK293T cells. ( A ) Agarose gel and ( B ) quantification of DNA cleavage by SaCas9. Graphs represent the mean of three replicates. Error bars: S.E.M. ( C ) HEK293Ts are transiently transfected with plasmids carrying SaCas9 + sgRNA. A second plasmid encodes either AcrIIA4 (positive control) or AcrIIA11. ( D ) Representative agarose gel and ( E ) quantification of indel percentage for the CACNA1D site. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; *** P < 0.001; **** P < 0.0001) were determined using a Student’s t -test. ( F ) Quantification of the editing efficiency when AcrIIA11 or AcrIIA4 are present relative to when no Acr is expressed. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; **** P < 0.0001) were determined using a Student’s t -test.

    Journal: Nucleic Acids Research

    Article Title: Mechanism of Cas9 inhibition by AcrIIA11

    doi: 10.1093/nar/gkaf318

    Figure Lengend Snippet: AcrIIA11 inhibits SaCas9 in vitro and in human HEK293T cells. ( A ) Agarose gel and ( B ) quantification of DNA cleavage by SaCas9. Graphs represent the mean of three replicates. Error bars: S.E.M. ( C ) HEK293Ts are transiently transfected with plasmids carrying SaCas9 + sgRNA. A second plasmid encodes either AcrIIA4 (positive control) or AcrIIA11. ( D ) Representative agarose gel and ( E ) quantification of indel percentage for the CACNA1D site. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; *** P < 0.001; **** P < 0.0001) were determined using a Student’s t -test. ( F ) Quantification of the editing efficiency when AcrIIA11 or AcrIIA4 are present relative to when no Acr is expressed. Error bars are the standard deviation of three replicates. P -values (not significant [ns], P > 0.05; **** P < 0.0001) were determined using a Student’s t -test.

    Article Snippet: The Sa Cas9 and CMV promoter-driven AcrIIA4 expression vectors were purchased from Addgene (Plasmid #85452 and #113038) [ , ].

    Techniques: In Vitro, Agarose Gel Electrophoresis, Transfection, Plasmid Preparation, Positive Control, Standard Deviation